Journal: The Kaohsiung Journal of Medical Sciences

Article Title: MFAP2 aggravates tumor progression through activating FOXM1 /β‐catenin‐mediated glycolysis in ovarian cancer

doi: 10.1002/kjm2.12546

Figure Lengend Snippet: MFAP2 expression was elevated in ovarian cancer cells and promoted the proliferation of ovarian cancer cells. (A) The mRNA expression of MFAP2 in human ovarian cancer cell lines (A2780, OVCAR4, CAOV3, TOV‐21G, SKOV3) and human normal ovarian epithelial cell line (IOSE80) was analyzed by qRT‐PCR assay; (B) the protein level of MFAP2 in human ovarian cancer cell lines (A2780, OVCAR4, CAOV3, TOV‐21G, SKOV3) and human normal ovarian epithelial cell line (IOSE80) was determined by western blot assay; (C) the mRNA expression of MFAP2 in A2780 and SKOV3 cells was measured by quantitative real‐time polymerase chain reaction assay; (D) the protein level of MFAP2 in A2780 and SKOV3 cells was determined by western blot assay; (E) CCK‐8 assay was used to determine the cell viabilities of A2780 and SKOV3 cells; (F) colony formation assay was used to detect the number of cell clones in A2780 and SKOV3 cells; (G) flow cytometry was used to detect cell cycle changes. Data are shown as mean ± SD; * p < 0.05, ** p < 0.01, and *** p < 0.001. CCK‐8, Cell Count Kit‐8; MFAP2, Microfibril‐associated protein 2; qRT‐PCR, xxxxx

Article Snippet: The human normal ovarian cell line IOSE80 was purchased from iCell Bioscience Inc. (Shanghai, China), while the ovarian cancer cell lines A2780, CAOV3, TOV‐21G, and SKOV3, were purchased from the American Type Culture Collection cell bank (Manassas, VA, USA); in addition, OVCAR4 was obtained from Cobioer lnc. (Nanjing, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Real-time Polymerase Chain Reaction, CCK-8 Assay, Colony Assay, Clone Assay, Flow Cytometry, Cell Counting

Journal: The Kaohsiung Journal of Medical Sciences

Article Title: MFAP2 aggravates tumor progression through activating FOXM1 /β‐catenin‐mediated glycolysis in ovarian cancer

doi: 10.1002/kjm2.12546

Figure Lengend Snippet: MFAP2 promotes glycolysis of ovarian cancer cells. (a–c) Glucose uptake(A), lactate production (B), and ATP synthesis (C) of A2780 and SKOV3 cells were determined by Glucose Uptake Colorimetric Assay Kit, Lactate Assay Kit II, and ATP Colorimetric Assay Kit. (D,E) The ECAR (D) and OCR(E) of A2780 and SKOV3 cells were measured using the Seahorse Extracellular Flux Analyzer XF96 analyzer. Data are shown as mean ± SD; * p < 0.05, ** p < 0.01, and *** p < 0.001. ECAR, extracellular acidification ratio; MFAP2, Microfibril‐associated protein 2; OCR, oxygen consumption ratio

Article Snippet: The human normal ovarian cell line IOSE80 was purchased from iCell Bioscience Inc. (Shanghai, China), while the ovarian cancer cell lines A2780, CAOV3, TOV‐21G, and SKOV3, were purchased from the American Type Culture Collection cell bank (Manassas, VA, USA); in addition, OVCAR4 was obtained from Cobioer lnc. (Nanjing, China).

Techniques: Colorimetric Assay, Lactate Assay

Journal: The Kaohsiung Journal of Medical Sciences

Article Title: MFAP2 aggravates tumor progression through activating FOXM1 /β‐catenin‐mediated glycolysis in ovarian cancer

doi: 10.1002/kjm2.12546

Figure Lengend Snippet: MFAP2 promotes FOXM1/β‐catenin‐mediated glycolysis signaling in ovarian cancer cells. (A) The levels of FOXM1 and glycolysis‐related protein expression in A2780 and SKOV3 cells were determined by western blot assay; (B) the levels of Wnt/β‐catenin signaling pathway and its downstream target genes in A2780 and SKOV3 cells were determined by western blot assay; (C) the levels of β‐catenin in the nucleus and cytoplasm of A2780 and SKOV3 cells were analyzed by western blot assay. Data are shown as mean ± SD; * p < 0.05, ** p < 0.01, and *** p < 0.001. MFAP2, Microfibril‐associated protein 2

Article Snippet: The human normal ovarian cell line IOSE80 was purchased from iCell Bioscience Inc. (Shanghai, China), while the ovarian cancer cell lines A2780, CAOV3, TOV‐21G, and SKOV3, were purchased from the American Type Culture Collection cell bank (Manassas, VA, USA); in addition, OVCAR4 was obtained from Cobioer lnc. (Nanjing, China).

Techniques: Expressing, Western Blot

Journal: The Kaohsiung Journal of Medical Sciences

Article Title: MFAP2 aggravates tumor progression through activating FOXM1 /β‐catenin‐mediated glycolysis in ovarian cancer

doi: 10.1002/kjm2.12546

Figure Lengend Snippet: Knockdown of MFAP2 inhibits xenograft tumor growth of ovarian cancer cells in vivo (A) photographs of tumors excised from the mice injected with SKOV3 cells transfected with shNC or shMFAP2. (B,C) Tumor growth curves (B) and tumor weight (C) of mice injected with A2780 cells. (D) The levels of MFAP2, Ki‐67, FOXM1, GLUT1, HK2, and β‐catenin in tumors from mice injected with SKOV3 cells were evaluated by immunohistochemical (IHC) staining (scale bar = 100 μm, magnification, ×200; scale bar = 50 μm, magnification, ×400). Data are shown as mean ± SD; * p < 0.05, ** p < 0.01, and *** p < 0.001. IHC, xxxxxx; MFAP2, Microfibril‐associated protein 2

Article Snippet: The human normal ovarian cell line IOSE80 was purchased from iCell Bioscience Inc. (Shanghai, China), while the ovarian cancer cell lines A2780, CAOV3, TOV‐21G, and SKOV3, were purchased from the American Type Culture Collection cell bank (Manassas, VA, USA); in addition, OVCAR4 was obtained from Cobioer lnc. (Nanjing, China).

Techniques: Knockdown, In Vivo, Injection, Transfection, Immunohistochemical staining, Immunohistochemistry